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Updated: Aug 14, 2026

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Published on: January 8, 2020
Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta
C M Serdar1, D T Gibson, D M Munnecke
1Center for Applied Microbiology, Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712 and Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019.
Abstract:
An organism identified as Pseudomonas diminuta was found to hydrolyze parathion. Cells grown for 48 h contained 3,400 U of parathion hydrolase activity per liter of broth. Expression of enzymatic activity was lost at a high frequency (9 to 12%) after treatment with mitomycin C. Hydrolase-negative derivatives were missing a plasmid present in the wild-type organism. The molecular mass of this plasmid (pCS1), as determined by electron microscopy, was about 44 x 10 daltons.
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