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A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
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Comparison of the Pour, Spread, and Drop Plate Methods for Enumeration of Rhizobium spp. in Inoculants Made from

H J Hoben1, P Somasegaran

  • 1NifTAL Project, Department of Agronomy and Soil Science, College of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, Hawaii 96822.

Applied and Environmental Microbiology
|November 1, 1982
PubMed

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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...