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Updated: Aug 14, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Nitrite and nitric oxide as inhibitors of nitrogenase from soybean bacteroids
1Laboratoire de Biologie végétale, Faculté des Sciences et des Techniques, Parc Valrose, 06034 Nice Cedex, France.
Abstract:
Nitrite was able to strongly inhibit C(2)H(2) reduction by nitrogenase from soybean bacteroids, whereas H(2) evolution was unaffected under the same conditions. NO inhibited both C(2)H(2) reduction and H(2) evolution; during C(2)H(2) reduction, sensitivity of nitrogenase to NO was higher than to NO(2), and the K(i) values were, respectively, 0.056 and 0.52 mM. Production of NO resulting from a reduction of NO(2) by dithionite in nitrogenase incubations was observed. However, the characteristics of inhibitions and the low level of NO generated by nitrite reduction ruled out the suggestion concerning a direct role of NO to explain the inhibitory effect of NO(2) on nitrogenase.
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