Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Congo Red Absorption by Rhizobium leguminosarum.

B E Kneen1, T A Larue

  • 1Boyce Thompson Institute for Plant Research, Ithaca, New York, 14853.

Applied and Environmental Microbiology
|January 1, 1983
PubMed
Summary

Rhizobium leguminosarum can absorb Congo red dye, contrary to common belief. This dye uptake varies by strain and may serve as a useful marker for identifying specific Rhizobium leguminosarum strains.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosomal location of lectin genes indicates they are not the basis of Rhizobium strain specificity mutations identified in pea (Pisum sativum L.).

The Journal of heredity·1994
Same author

Exogenous Ethylene Inhibits Nodulation of Pisum sativum L. cv Sparkle.

Plant physiology·1992
Same author

Pleiotropic Effects of sym-17 : A Mutation in Pisum sativum L. cv Sparkle Causes Decreased Nodulation, Altered Root and Shoot Growth, and Increased Ethylene Production.

Plant physiology·1992
Same author

Ethylene as a Possible Mediator of Light- and Nitrate-Induced Inhibition of Nodulation of Pisum sativum L. cv Sparkle.

Plant physiology·1992
Same author

Ethylene Inhibitors Partly Restore Nodulation to Pea Mutant E 107 (brz).

Plant physiology·1992
Same author

Light Microscopy Study of Nodule Initiation in Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5).

Plant physiology·1991

Area of Science:

  • Microbiology
  • Bacteriology

Background:

  • Congo red absorption is typically viewed as a negative indicator for Rhizobium species.
  • This dye is often used in bacterial identification and characterization.

Purpose of the Study:

  • To investigate the Congo red absorption capabilities of Rhizobium leguminosarum.
  • To determine if Congo red uptake can be a useful characteristic for differentiating strains within Rhizobium leguminosarum.

Main Methods:

  • Culturing Rhizobium leguminosarum strains on yeast extract-mannitol agar containing Congo red.
  • Observing and comparing the degree of Congo red uptake among different strains.

Main Results:

  • Rhizobium leguminosarum strains demonstrated the ability to absorb Congo red dye.
  • Significant variation in Congo red uptake was observed among different R. leguminosarum strains.
  • Congo red absorption did not reliably differentiate Rhizobium from other bacteria.

Conclusions:

  • Congo red absorption is not a universal contraindication for all Rhizobium species.
  • The variability in dye uptake suggests its potential utility as a strain-specific marker for Rhizobium leguminosarum.
  • Further research may refine the use of Congo red for Rhizobium strain identification.

Related Experiment Videos