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Replica plating method for estimating phenanthrene-utilizing and phenanthrene-cometabolizing microorganisms.

M P Shiaris1, J J Cooney

  • 1Chesapeake Biological Laboratory, Center for Environmental and Estuarine Studies, and Department of Microbiology, University of Maryland, Solomons, Maryland 20668.

Applied and Environmental Microbiology
|February 1, 1983
PubMed
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A new replica plating method identifies microorganisms that degrade phenanthrene. This technique distinguishes between organisms that use phenanthrene for energy and those that degrade it alongside other compounds, revealing their coexistence in estuarine environments.

Area of Science:

  • Environmental microbiology
  • Bioremediation

Background:

  • Phenanthrene is a polycyclic aromatic hydrocarbon pollutant.
  • Microbial degradation is a key process for removing phenanthrene from the environment.

Purpose of the Study:

  • To develop a novel replica plating method for quantifying phenanthrene-degrading microorganisms.
  • To differentiate between microorganisms that utilize phenanthrene as a sole carbon source and those that cometabolize it.

Main Methods:

  • Development of a selective replica plating technique.
  • Enumeration of phenanthrene-degrading microbial populations in estuarine sediments.

Main Results:

  • A replica plating method was successfully established.
  • The method effectively distinguished between phenanthrene utilizers and cometabolizers.

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  • Both types of phenanthrene-degrading microorganisms were found to coexist in estuarine sediments.

    • Conclusions:

    • The developed replica plating method is effective for studying phenanthrene-degrading microorganisms.
    • Estuarine sediments harbor diverse microbial communities capable of phenanthrene degradation.
    • Understanding these microbial populations is crucial for bioremediation strategies.