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Published on: January 6, 2015
Ability of Daphnia Cell-Free Extract to Damage Escherichia coli Cells
1Kinneret Limnological Laboratory, Tiberias, 14-102 Israel, The Technion, Israel Institute of Technology, Haifa, 32000 Israel, and Department of Molecular Biology, Hadassa Medical School, The Hebrew University, Jerusalem, 91000 Israel.
Abstract:
A cell-free extract of Daphnia magna was found to lyse Escherichia coli cells as shown by leakage of the enzymes alkaline phosphatase and beta-galactosidase from the bacteria. The cell-free extract was separated on Sephadex G-200, and the fractions showing an ability to lyse E. coli cels were isolated. The factor which was responsible for the lysis of the bacterial cells was probably a protein with a molecular weight of several thousands. Mg and Ca ions augmented the activity of the Daphnia extract on E. coli cells.
