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Wide-host-range plasmids function in the genus thiobacillus.

M S Davidson1, A O Summers

  • 1Department of Microbiology, University of Georgia, Athens, Georgia 30602.

Applied and Environmental Microbiology
|September 1, 1983
PubMed
Summary
This summary is machine-generated.

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Plasmids can be transferred to Thiobacillus novellus from Escherichia coli, enabling genetic marker expression. However, plasmid transfer to other Thiobacillus species is limited, though they can transfer plasmids back to E. coli.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Plasmids are crucial for horizontal gene transfer in bacteria.
  • Thiobacillus species are important in various industrial and environmental processes.
  • Understanding plasmid transfer in Thiobacillus is key for genetic manipulation.

Purpose of the Study:

  • To investigate the transmissibility of various plasmids between Escherichia coli and different Thiobacillus species.
  • To assess the stability and expression of genetic markers from plasmids in Thiobacillus hosts.
  • To explore the potential of using plasmids as cloning vectors in Thiobacillus.

Main Methods:

  • Bacterial conjugation experiments were performed using various plasmids (S-a, RP4, R388, pMD101, pDT387, pDT566, pVK100, R300B) and helper plasmids (pRK2013).

Related Experiment Videos

  • Recipient strains included Thiobacillus novellus, T. intermedius, T. perometabolis, T. neapolitanus, and T. acidophilus.
  • Genetic marker expression (chloramphenicol and ampicillin resistance) and plasmid stability were evaluated.
  • Donor-specific bacteriophage sensitivity was tested.

    • Main Results:

    • Plasmids RP4 and its derivatives were successfully transferred to Thiobacillus novellus, with most genetic markers expressed.
    • Plasmid transfer from E. coli to other Thiobacillus species was generally unsuccessful, except when mediated by T. novellus.
    • Most Thiobacillus species could transfer plasmids back to E. coli, with T. acidophilus being an exception.
    • The cosmid cloning vehicle pVK100 was mobilized to T. novellus and found to be stable, while the helper plasmid pRK2013 was not.
    • Native plasmids were identified in T. intermedius and T. acidophilus.

    Conclusions:

    • Plasmid conjugation efficiency varies significantly between Escherichia coli and different Thiobacillus species.
    • Thiobacillus novellus serves as a potential intermediate host for transferring plasmids to other Thiobacillus species.
    • Plasmids like pVK100 show promise for genetic engineering applications in Thiobacillus.
    • The presence of native plasmids in some Thiobacillus species warrants further investigation.