Related Experiment Video
Updated: Aug 14, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Kinetics of Sulfate and Acetate Uptake by Desulfobacter postgatei
K Ingvorsen1, A J Zehnder, B B Jørgensen
1Swiss Federal Institute for Water Resources and Water Pollution Control, Swiss Federal Institutes of Technology, CH-8600 Dübendorf, Switzerland, and Institute of Ecology and Genetics, University of Aarhus, DK-8000 Aarhus C, Denmark.
Abstract:
The kinetics of sulfate and acetate uptake was studied in the sulfate-reducing bacterium Desulfobacter postgatei (DSM 2034). Kinetic parameters (K(m) and V(max)) were estimated from substrate consumption curves by resting cell suspensions with [S]sulfate and [C]acetate. Both sulfate and acetate consumption followed Michaelis-Menten saturation kinetics. The half-saturation constant (K(m)) for acetate uptake was 70 muM with cells from either long-term sulfate- or long-term acetate-limited chemostat cultures. The average K(m) value for sulfate uptake by D. postgatei was about 200 muM. K(m) values for sulfate uptake did not differ significantly when determined with cells derived either from batch cultures or sulfate- or acetate-limited chemostat cultures. Acetate consumption was observed at acetate concentrations of =1 muM, whereas sulfate uptake usually ceased at 5 to 20 muM. The results show that D. postgatei is not freely permeable to sulfate ions and further indicate that sulfate uptake is an energy-requiring process.
More Related Videos
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
11:58Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
Published on: December 29, 2013
Related Concept Videos
Sulfur Assimilation
Microbes and the Sulfur Cycle
Microbial Nutrition
Lipid Catabolism
Microbes and Methanogenesis
Carbon-dioxide Fixation