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Improved double-vial radiorespirometric technique for mineralization of C-labeled substrates
1W. K. Kellogg Biological Station, Michigan State University, Hickory Corners, Michigan 49060.
Applied and Environmental Microbiology
|May 1, 1984
Abstract:
New procedures which simplify sample preparation and improve counting efficiency were developed for double-vial radiorespirometry. Under certain conditions, efficiency of fluor-NaOH-impregnated wicks was not stable, but was adversely affected by water vapor, NaOH concentration, and CO(2) loading. Glass fiber wick material treated with a methanolic fluor-NaOH solution showed improved C counting efficiency (58%) compared with previous methods.

