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General method for the identification of plasmid species in fast-growing soil microorganisms
J Plazinski1, Y H Cen, B G Rolfe
1Department of Genetics, Research School of Biological Sciences, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
Abstract:
Using a horizontal gel electrophoresis method, we demonstrated reproducibly the presence of indigenous plasmids in different Rhizobium, Agrobacterium, and Pseudomonas strains. The method yields a large amount of plasmid DNA and is sensitive in detecting megaplasmids with molecular weights higher than 5 x 10. In two Rhizobium meliloti strains, a megaplasmid other than the low-mobility plasmid already known was detected.
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