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Use of the mushroom tissue block rapid pitting test to detect brown blotch pathogens
1Botany Department, University of Maine, Orono, Maine 04469.
Abstract:
Isolates of an Arthrobacter species known to produce a positive host pathogenicity test were evaluated by the mushroom tissue block rapid pitting test. All 11 isolates of the Arthrobacter species yielded a positive test response. The time required for a positive test was the same for Pseudomonas tolaasi and the Arthrobacter species.
Insights
This study found that all tested Arthrobacter isolates showed a positive rapid pitting test, indicating pathogenicity. The reaction time was comparable to Pseudomonas tolaasi, a known mushroom pathogen.
Area of Science:
- Microbiology
- Plant Pathology
- Mycology
Background:
- Certain Arthrobacter species are known to cause disease in hosts.
- The mushroom tissue block rapid pitting test is a method to assess pathogenicity.
Purpose of the Study:
- To evaluate the pathogenicity of Arthrobacter species using the rapid pitting test.
- To compare the efficacy of the rapid pitting test for Arthrobacter with a known pathogen, Pseudomonas tolaasi.
Main Methods:
- Eleven isolates of Arthrobacter species were subjected to the mushroom tissue block rapid pitting test.
- The time taken for a positive result was recorded and compared.
Main Results:
- All eleven Arthrobacter isolates tested positive in the rapid pitting assay.
- The time to achieve a positive result for Arthrobacter was similar to that observed for Pseudomonas tolaasi.
Conclusions:
- The rapid pitting test is effective in identifying pathogenic Arthrobacter species.
- Arthrobacter species exhibit a similar pathogenic response time to Pseudomonas tolaasi in this assay.
