Selective Isolation of Bacillus sphaericus from Soil by Use of Acetate as the Only Major Source of Carbon
Related Experiment Video
Updated: Aug 14, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
J Massie1, G Roberts, P J White
1Department of Microbiology, The University, Western Bank, Sheffield S10 2TN, United Kingdom.
Abstract:
A simple chemically defined medium containing sodium acetate (37 or 74 mM) as the only major source of carbon was inoculated with soil from various locations. The bacteria which grew most rapidly at 30 degrees C were almost entirely strains of Bacillus sphaericus and Arthrobacter species. Pasteurization of the soils made the medium selective for B. sphaericus. Several new strains were isolated, and the peptidoglycans of their cell walls were examined.
More Related Videos
Related Concept Videos
Production of Organic Acids
Production of Biopesticides
Techniques for Isolation of Pure Cultures
Microbes in the Production of Fermented Foods

