Related Experiment Video
Updated: Aug 14, 2026

Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
Published on: September 6, 2024
Effect of gramicidin on methanogenesis by various methanogenic bacteria
1Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
Abstract:
Methanogenesis by Methanobacterium thermoautotrophicum strains was extremely sensitive to gramicidin, total inhibition being observed at 0.2 mug/ml. In contrast, methane synthesis by Methanococcus voltae, Methanogenium marisnigri, Methanosarcina mazei, and Methanospirillum hungatei were resistant to the highest concentrations of gramicidin tested (40 mug/ml), although spheroplasts of Methanospirillum hungatei were extremely sensitive. Other species tested showed intermediate sensitivity to gramicidin, methanogenesis inhibition occurring at 4 to 20 mug/ml.
Related Concept Videos
Microbes and Methanogenesis
Microbial Interactions: Mutualism
Overview of Archaea
Metabolism of Chemolithotrophs
Amino Acid Catabolism
Archaeal Cell Wall

