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Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
Biochemical and immunological study of cell envelope proteins in sulfate-reducing bacteria
1Division of Microbiology, National Defence Research Institute, S-901 82 Umeå, Sweden.
Abstract:
The envelope proteins of 5 strains of the genus Desulfotomaculum and 12 strains of the genus Desulfovibrio were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The Desulfovibrio strains exhibited a typical gram-negative cell envelope, whereas the cell envelope of Desulfotomaculum strains appeared to be gram-positive. A close relationship between strains of Desulfotomaculum nigrificans was observed. A comparison between different species of Desulfotomaculum revealed some degree of similarity between Desulfotomaculum nigrificans and Desulfotomaculum ruminis, whereas Desulfotomaculum orientis seemed unique. The strains of Desulfovibrio salexigens were quite different from the strains of the other species of Desulfovibrio. In two of the strains of Desulfovibrio desulfuricans, a species-specific antigen was observed. The strains of Desulfovibrio vulgaris, Desulfovibrio africanus, and Desulfovibrio gigas and one strain of Desulfovibrio desulfuricans exhibited a similar outer membrane protein profile and also showed very similar antigenic reactions.
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