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Published on: January 15, 2014
Isolation of Gregatin A from Phialophora gregata by Preparative High-Pressure Liquid Chromatography
S L Taylor1, R E Peterson, L E Gray
1Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Plant Pathology, Agricultural Research Service, U.S. Department of Agriculture, Urbana, Illinois 61801.
Abstract:
A method was developed for the production and purification of gregatin A from Phialophora gregata NRRL 13198 cultured on rice at 20 degrees C for 28 days. Liquid extraction followed by high-pressure liquid chromatography afforded 247.0 mg of crystalline gregatin A per kg of rice.
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