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Related Experiment Videos

Diffusion through a Double-Sided Plate: Development of a Method to Study Alga-Bacterium Interactions.

F S Colwell1, H K Speidel

  • 1Department of Biology, Northern Arizona University, Flagstaff, Arizona 86011.

Applied and Environmental Microbiology
|December 1, 1985
PubMed
Summary

This study explored microbial interactions using double-layer agar plates. Bacteria and algae showed altered growth, indicating the plates effectively screen interactions via diffusing extracellular products.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Algology

Background:

  • Wastewater oxidation ponds harbor diverse microbial communities, including bacteria and algae.
  • Understanding microbial interactions is crucial for wastewater treatment and ecosystem health.

Purpose of the Study:

  • To evaluate the utility of double-layer agar plates (Lutri plates) for studying interactions between bacteria and algae.
  • To determine if extracellular products diffuse through agar and influence microbial growth.

Main Methods:

  • Bacteria and algae from wastewater were co-cultured on opposing surfaces of double-layer agar plates.
  • Algal growth characteristics were observed in response to adjacent bacterial cultures.

Main Results:

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  • Algal growth patterns were significantly altered by the presence of different bacterial species on the adjacent agar layer.
  • Observed growth alterations suggest the diffusion of bacterial extracellular products through the agar medium.

Conclusions:

  • Double-layer agar plates provide a simple and effective method for screening interactions between actively growing microorganisms.
  • This technique facilitates the study of microbial consortia in environmental samples like wastewater.