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Related Experiment Videos

Large plasmids from soil bacteria enriched on halogenated alkanoic acids.

D J Hardman1, P C Gowland, J H Slater

  • 1Department of Environmental Sciences, University of Warwick, Coventry CV4 7AL, West Midlands, United Kingdom.

Applied and Environmental Microbiology
|January 1, 1986
PubMed
Summary

Soil bacteria, including Pseudomonas and Alcaligenes species, possess large plasmids enabling growth on halogenated acids and conferring heavy metal resistance. Plasmid pUU204 showed instability and gene transfer capabilities.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Molecular Biology

Background:

  • Soil microorganisms exhibit diverse metabolic capabilities.
  • Halogenated alkanoic acids are environmental pollutants.
  • Plasmids are extrachromosomal DNA elements that can confer advantageous traits.

Purpose of the Study:

  • To isolate and characterize soil bacteria capable of degrading halogenated alkanoic acids.
  • To investigate the role of plasmids in conferring this degradative ability and heavy metal resistance.
  • To assess the stability and transferability of a specific plasmid, pUU204.

Main Methods:

  • Isolation of bacterial strains from soil.
  • Plasmid DNA isolation and molecular weight determination.
  • Growth studies using halogenated alkanoic acids as sole carbon sources.

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  • Heavy metal resistance assays.
  • Plasmid stability experiments in continuous culture.
  • Gene transfer experiments using a conjugative plasmid.

    • Main Results:

    • Four Pseudomonas and two Alcaligenes species were isolated, all containing large plasmids (98,800–190,000 MW).
    • These plasmids were associated with the utilization of 2-monochloropropionic acid and 2-monochloroacetic acid, and resistance to mercury, selenium, or tellurium.
    • The largest plasmid, pUU204, was unstable in continuous culture with succinate as the carbon source.
    • The dehalogenase gene from pUU204 was transferable to the IncP plasmid R68-45.

    Conclusions:

    • Large plasmids play a significant role in the ability of soil bacteria to degrade halogenated alkanoic acids and resist heavy metals.
    • Plasmid pUU204 is unstable under specific conditions but harbors a transferable dehalogenase gene.
    • These findings have implications for bioremediation strategies using microbial communities.