Characterization of Pseudomonas maltophilia Isolates from Fleece Rot

J A Macdiarmid1, D H Burrell

  • 1Commonwealth Scientific and Industrial Research Organisation, Division of Animal Health, McMaster Laboratory, Glebe, New South Wales 2037, Australia.

Insights

Pseudomonas maltophilia isolates from sheep with fleece rot showed biochemical similarities to human strains. This suggests a potential link between this bacterium and ovine fleece discoloration.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Sheep Health

Background:

  • Pseudomonas maltophilia is a bacterium found in various environments.
  • Fleece rot in sheep causes significant economic losses.
  • The specific role of P. maltophilia in ovine fleece rot is not fully understood.

Purpose of the Study:

  • To investigate the biochemical characteristics of Pseudomonas maltophilia isolates from sheep with fleece rot.
  • To compare the enzymatic profiles of ovine P. maltophilia with human strains.
  • To explore the potential association between P. maltophilia and ovine fleece rot.

Main Methods:

  • Collection of 286 ovine isolates of Pseudomonas maltophilia from affected sheep.
  • Enzyme activity analysis of 10 isolates using plate tests.
  • Utilizing the API ZYM system for detailed enzymatic profiling.
  • Comparison with the enzymatic profile of a human P. maltophilia type strain.

Main Results:

  • Ovine P. maltophilia isolates exhibited biochemical similarity to human strains.
  • Enzymatic profiles were comparable between ovine and human isolates.
  • The study identified specific enzyme activities in the ovine isolates.

Conclusions:

  • Ovine Pseudomonas maltophilia strains are biochemically similar to pathogenic human strains.
  • This biochemical similarity suggests a potential association between P. maltophilia and brown to yellow fleece rot in sheep.
  • Further research is warranted to confirm the pathogenic role of ovine P. maltophilia in fleece rot.