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Updated: Aug 14, 2026

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
Nitrogen Limitation in Natural Populations of Cyanobacteria (Spirulina and Oscillatoria spp.) and Its Effect on
1The Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Abstract:
Natural populations of the cyanobacteria Spirulina species and Oscillatoria species obtained from Israeli fishponds were limited in growth by nitrogen availability in summer. Physiological indicators for nitrogen limitation, such as phycocyanin, chlorophyll a, and carbohydrate content, did not show clear evidence for nitrogen limited growth, since these organisms are capable of vertical migration from and to the nitrogen-rich bottom. By means of C labeling of the cells under simulated pond conditions followed by cell fractionation into macromolecular compounds, we found that carbohydrates synthesized at the lighted surface were partially utilized for dark protein synthesis at the bottom of these ponds.
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