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Related Experiment Videos

Denitrification by Chromobacterium violaceum.

D A Bazylinski1, E Palome, N A Blakemore

  • 1Department of Microbiology, University of New Hampshire, Durham, New Hampshire 03824.

Applied and Environmental Microbiology
|October 1, 1986
PubMed
Summary

Chromobacterium violaceum strains ferment glucose anaerobically and reduce nitrate, primarily producing nitrous oxide (N2O). This N2O production is not linked to growth but may detoxify nitrite.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Biochemistry

Background:

  • Chromobacterium violaceum is a Gram-negative bacterium found in various environments.
  • Understanding its metabolic capabilities, particularly under anaerobic conditions, is crucial for ecological and biotechnological applications.

Purpose of the Study:

  • To investigate the anaerobic growth and metabolic pathways of Chromobacterium violaceum strains.
  • To determine the role of nitrate and nitrite in the anaerobic metabolism of C. violaceum.

Main Methods:

  • Isolation and characterization of C. violaceum strains from freshwater habitats.
  • Anaerobic growth experiments using glucose as the sole carbon and energy source.
  • Analysis of fermentation products and nitrogenous compounds using gas chromatography and other biochemical assays.

Related Experiment Videos

  • Acetylene inhibition technique to study nitrogen fixation and N2O reduction.

    • Main Results:

    • C. violaceum strains grew anaerobically on glucose, producing acetate, propionate, lactate, and pyruvate.
    • Addition of sodium nitrate (NaNO3) significantly enhanced growth yield and rate.
    • Nitrate reduction yielded nitrite (NO2), nitric oxide (NO), and nitrous oxide (N2O), with N2O being the predominant end product (85-98%).
    • Nitrite did not support growth as effectively as nitrate, and its reduction to N2O was not coupled to growth.

    Conclusions:

    • C. violaceum ferments glucose anaerobically and can utilize nitrate as an electron acceptor, terminating denitrification with N2O production.
    • Nitrite reduction to N2O appears to be a detoxification mechanism rather than a growth-coupled process.
    • The strains did not exhibit nitrogen fixation activity.