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Ethanol Perturbs Glycosylation and Inhibits Hypersecretion in Trichoderma reesei
H Merivuori1, B S Montenecourt, J A Sands
1Department of Biology, Lehigh University, Bethlehem, Pennsylvania 18015.
Abstract:
The effects of ethanol and phenylethanol on the growth of and glycoprotein secretion by Trichoderma reesei were studied. Low levels (1.5%, vol/vol) of ethanol perturbed the glycosylation process, as shown by alterations in the isoelectric profile of the secreted proteins and a reduction in the rate of incorporation of mannose into oligosaccharides. In addition to these effects on posttranslational modification, ethanol drastically lowered the protein secretion level of a hypersecretory strain.
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