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Related Experiment Videos

Citrate Metabolism by Pediococcus halophilus.

C Kanbe1, K Uchida

  • 1Soy Sauce Research Laboratories, Kikkoman Corporation, Noda-shi, Chiba-ken 278, Japan.

Applied and Environmental Microbiology
|June 1, 1987
PubMed
Summary
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Soy pediococci that cannot metabolize citrate lack citrate lyase activity. Pediococcus halophilus utilizes citrate lyase to produce acetate and formate, with glucose reducing formate production.

Area of Science:

  • Microbiology
  • Food Science
  • Biochemistry

Background:

  • Soy sauce fermentation involves Pediococcus halophilus.
  • Some strains of P. halophilus do not metabolize citrate.
  • Citrate metabolism in these bacteria is not fully understood.

Purpose of the Study:

  • Investigate factors controlling citrate metabolism in soy pediococci.
  • Characterize the enzyme responsible for citrate decomposition.
  • Elucidate the metabolic pathway of citrate in P. halophilus.

Main Methods:

  • Enzyme assays for citrate lyase activity.
  • pH optima determination for citrate lyase.
  • Analysis of metabolic products from citrate using P. halophilus 7117 (Cit).

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Main Results:

  • Non-citrate-metabolizing strains lacked citrate lyase activity.
  • Citrate lyase in P. halophilus is an inducible enzyme with optimal activity at pH 7.0.
  • P. halophilus converts citrate to acetate and formate, lacking acetoin/diacetyl production; glucose inhibits formate production.

Conclusions:

  • Citrate lyase is essential for citrate decomposition in P. halophilus.
  • The citrate metabolism pathway in P. halophilus differs from lactic streptococci.
  • P. halophilus 7117 (Cit) possesses all necessary enzymes for converting citrate to acetate and formate.