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Related Experiment Videos

Isozyme Variation among 40 Frankia Strains.

M Gardes1, J Bousquet, M Lalonde

  • 1Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géodésie, Université Laval, Ste-Foy, Québec, Canada G1K 7P4.

Applied and Environmental Microbiology
|July 1, 1987
PubMed
Summary

Isozyme electrophoresis revealed significant variation among Frankia strains, aiding in the identification and grouping of these nitrogen-fixing bacteria based on host specificity groups like Alnus and Elaeagnus.

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Area of Science:

  • Microbiology
  • Plant Science
  • Biochemistry

Background:

  • Frankia strains are symbiotic nitrogen-fixing bacteria crucial for plant growth.
  • Understanding Frankia diversity is essential for optimizing symbiotic relationships.

Purpose of the Study:

  • To characterize Frankia strains using isozyme electrophoresis.
  • To assess genetic diversity within and between host specificity groups (Alnus and Elaeagnus).

Main Methods:

  • Electrophoretic separation of isozymes from eight enzymes.
  • Analysis of enzyme variations in 40 Frankia strains.
  • Cluster analysis based on similarity coefficients.

Main Results:

  • High variability observed across most enzyme systems, with diaphorases and esterases showing multiple bands.

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  • Phosphoglucose isomerase, leucine aminopeptidase, and malate dehydrogenase revealed larger groupings.
  • Cluster analysis delineated three dissimilar Frankia strain groups with internal heterogeneity.
  • Many Alnus-specific strains showed perfect homology across all tested enzymes.

    • Conclusions:

    • Isozyme electrophoresis is effective for differentiating Frankia strains.
    • Frankia populations exhibit significant genetic diversity, with distinct clustering patterns.
    • Homology within the Alnus group suggests potential for specific strain selection.