Determining [H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment
P Servais1, J Martinez, G Billen
1Groupe de Microbiologie des Milieux Aquatiques, Université Libre de Bruxelles, 1050 Brussels, Belgium, and Laboratorio de Microbiologia, Facultad de Biologia, Av. Diagonal 645, 08028 Barcelona, Spain.
Abstract:
Determination of [H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method, the fraction of thymidine incorporated into DNA ranged from 10 to 83%.
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