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Related Experiment Videos

A Positive Strain Identification Method for Rhizobium meliloti.

R Wheatcroft1, R J Watson

  • 1Plant Research Centre, Agriculture Canada, Ottawa, Ontario K1A 0C6, Canada.

Applied and Environmental Microbiology
|February 1, 1988
PubMed
Summary

Rhizobium meliloti strains can be identified by analyzing insertion sequence ISRm1 copy numbers and DNA hybridization patterns. This method helps determine strain identity and relatedness, even with minor genomic changes.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Insertion sequence ISRm1 is present in 80% of Rhizobium meliloti strains, with 1 to 11 copies per genome.
  • DNA hybridization patterns using an ISRm1-specific probe are unique to each strain.

Purpose of the Study:

  • To establish a method for identifying Rhizobium meliloti strains based on ISRm1 insertion sequences.
  • To assess the utility of ISRm1 patterns for determining strain identity and relatedness.

Main Methods:

  • Analyzing the number of ISRm1 copies in Rhizobium meliloti genomes.
  • Performing DNA hybridization with an ISRm1-specific probe on separated genomic DNA fragments.
  • Comparing hybridization band patterns between different strains.

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Main Results:

  • Each Rhizobium meliloti strain exhibits a distinctive hybridization pattern.
  • These patterns allow for the differentiation of strains.
  • Strain relatedness can be inferred from hybridization patterns, even with phenotypic or minor genomic variations.

Conclusions:

  • Insertion sequence ISRm1-based DNA hybridization is a reliable method for Rhizobium meliloti strain identification.
  • This technique is valuable for confirming or refuting common strain identity.
  • The method effectively infers relatedness among strains, accommodating minor genomic alterations.