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Cell-Free Protein Synthesis System for Building Synthetic Cells
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Preparation of a Cell-Free, Isobutene-Forming System from Rhodotorula minuta
1Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860, Japan.
Applied and Environmental Microbiology
|February 1, 1988
Abstract:
The cell-free isobutene-forming system of Rhodotorula minuta IFO 1102 was prepared. This system consisted of isovaleric acid, NADPH, and a cell extract. The maximal activity obtained by using the described cell-free system was 17 nl/mg of protein per h. It appears that this cell-free isobutene-forming system operates in living cells of R. minuta.
