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Updated: Aug 14, 2026
Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
One-Step Purification of a Lipolytic Enzyme from Bacillus pumilus by Mild Hydrophobic Interaction Chromatography on
1Laboratoire de Chimie-Physique Macromoléculaire, Ecole Nationale Supérieure des Industries Chimiques, 1, Rue Grandville, 54042 Nancy Cedex, and Laboratoire de Microbiologie, Ecole Supérieure des Sciences et Technologies des Industries du Bois, Université de Nancy I, 54506 Vandoeuvre-lés-Nancy Cedex, France.
Abstract:
An esterase from Bacillus pumilus was obtained in a highly purified form, starting from a crude extract, by a new chromatographic technique requiring no detergent during the elution step. The stationary phase involved was mildly hydrophobic and was prepared by covalent immobilization of a polyoxyalkylene glycol onto Sepharose 6B.
