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Genetic Transformation of an argB Mutant of Aspergillus oryzae
Applied and Environmental Microbiology
|June 1, 1988
Abstract:
An argB mutant of Aspergillus oryzae NRRL 492 has been genetically transformed with the Aspergillus nidulans argB gene. Protoplasts were generated with a combination of Novozyme 234 and beta-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for A. nidulans. A frequency of 5 to 10 transformants per mug of DNA was obtained; however, most transformants appeared abortive. The A. nidulans argB gene and vector sequences appeared to be integrated into the A. oryzae chromosome.
