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Related Experiment Videos

Combined oxygen and nitrous oxide microsensor for denitrification studies.

N P Revsbech1, L P Nielsen, P B Christensen

  • 1Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, DK-8000 Aarhus C, Denmark.

Applied and Environmental Microbiology
|September 1, 1988
PubMed
Summary

A new microsensor enables simultaneous measurement of oxygen (O2) and nitrous oxide (N2O). This tool accurately detects N2O in biofilms, revealing denitrification activity in anoxic layers.

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Microbiology

Background:

  • Simultaneous measurement of oxygen (O2) and nitrous oxide (N2O) is crucial for understanding microbial processes in various environments.
  • Existing methods may lack the sensitivity or specificity required for detailed microscale analysis in biofilms and sediments.

Purpose of the Study:

  • To describe the construction and performance of a novel microsensor for simultaneous O2 and N2O measurement.
  • To assess the microsensor's applicability in analyzing microprofiles within biofilms and sediments.
  • To investigate denitrification activity in biofilms using the developed microsensor.

Main Methods:

  • Construction of a microsensor with a tip diameter of 20 µm for simultaneous O2 and N2O detection.
  • Characterization of the microsensor's linear response, selectivity, and detection limits.

Related Experiment Videos

  • Measurement of O2 and N2O microprofiles in a biofilm treated with acetylene to inhibit N2O reductase.

    • Main Results:

    • The microsensor demonstrated a linear response to both O2 and N2O, with independent measurements.
    • A detection limit of approximately 1 µmol/L for N2O was achieved.
    • Acetylene treatment significantly increased N2O concentration in deeper biofilm layers, indicating denitrification activity.

    Conclusions:

    • The developed microsensor is a valuable tool for simultaneous O2 and N2O measurements in environmental samples.
    • The microsensor successfully detected denitrification activity in anoxic layers of biofilms.
    • Hydrogen sulfide was identified as a potential interfering agent, while other tested agents did not interfere.