Related Experiment Videos
Improved Assay for Rhodanese in Thiobacillus spp
1School of Life and Health Sciences, University of Delaware, Newark, Delaware 19716.
Applied and Environmental Microbiology
|November 1, 1988
Summary
This study introduces a refined method for assaying rhodanese (thiosulfate:cyanide sulfurtransferase) activity. By incorporating a boiled control, the new procedure accurately accounts for nonbiological product formation, improving assay reliability.
Area of Science:
- Biochemistry
- Enzymology
Background:
- Rhodanese (EC 2.8.1.1) catalyzes cyanide detoxification.
- Conventional rhodanese assays measure product formation colorimetrically.
- Nonbiological product formation complicates existing assay methods.
Purpose of the Study:
- To develop a modified rhodanese assay procedure.
- To improve the accuracy of rhodanese activity measurement.
- To correct for nonbiological product formation in rhodanese assays.
Main Methods:
- A modified assay procedure for rhodanese was developed.
- Each assay trial included a separate boiled control.
- Product formation was measured colorimetrically.
Main Results:
- The modified procedure corrects for nonbiological product formation.
- The boiled control accounts for spontaneous product generation.
- This enhances the accuracy of rhodanese activity determination.
Conclusions:
- The modified rhodanese assay provides a more accurate measure of enzymatic activity.
- This method is crucial for reliable biochemical studies involving rhodanese.
- Accurate rhodanese quantification is essential for understanding cyanide metabolism.