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Plasmid incidence in bacteria from deep subsurface sediments.

J K Fredrickson1, R J Hicks, S W Li

  • 1Pacific Northwest Laboratory, Richland, Washington 99352.

Applied and Environmental Microbiology
|December 1, 1988
PubMed
Summary
This summary is machine-generated.

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Subsurface bacteria in South Carolina sediments harbor plasmids and exhibit antibiotic resistance, particularly to penicillin and ampicillin. This resistance is linked to plasmid presence but not transferable, suggesting unique genetic adaptations in this environment.

Area of Science:

  • Microbiology
  • Environmental Science
  • Genetics

Background:

  • Deep terrestrial subsurface environments harbor unique microbial communities.
  • Antibiotic resistance is a growing global concern, necessitating investigation into diverse microbial habitats.
  • Plasmids play a crucial role in the dissemination of antibiotic resistance genes.

Purpose of the Study:

  • To characterize antibiotic and metal resistance profiles of bacteria from deep subsurface sediments.
  • To investigate the presence and role of plasmid DNA in conferring resistance.
  • To explore the potential for catabolic genes within these subsurface bacterial populations.

Main Methods:

  • Isolation and cultivation of bacteria from South Carolina subsurface sediments, surface soil, and drill muds.

Related Experiment Videos

  • Determination of antibiotic resistance using disk diffusion assays and minimum inhibitory concentrations (MICs).
  • Plasmid DNA analysis, DNA-hybridization studies using a TOL-plasmid probe, and metal MIC determination.

    • Main Results:

    • 33% of subsurface bacterial isolates contained plasmid DNA.
    • High frequencies of resistance observed for penicillin (70%), ampicillin (49%), and carbenicillin (32%).
    • Plasmid-bearing isolates showed significantly higher resistance to penicillin and ampicillin, though resistance was not transferable.

    Conclusions:

    • Subsurface bacteria possess intrinsic antibiotic resistance, partly mediated by plasmids.
    • Homology with TOL-plasmid DNA suggests potential for catabolic gene presence.
    • Distinct resistance patterns differentiate subsurface bacteria from drill mud contaminants.