Related Experiment Video
Updated: Aug 14, 2026

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Published on: December 19, 2011
Temperature Compensation in Methanosarcina barkeri by Modulation of Hydrogen and Acetate Affinity
P Westermann1, B K Ahring, R A Mah
1Environmental and Occupational Health Sciences, School of Public Health, University of California, Los Angeles, California 90024.
Abstract:
The affinity of Methanosarcina barkeri 227 for acetate and hydrogen at different incubation temperatures was investigated. Increasing the temperature from 20 to 37 degrees C resulted in a 4.5-fold increase in K(m) for acetate and a 4.8-fold increase for hydrogen. The corresponding increase in V(max) for acetate was 8.3-fold (5.4-fold for hydrogen). This response implied a decrease in the temperature coefficient (Q(10)) and hence a decrease in the temperature dependency as a function of decreasing substrate concentration.
More Related Videos
Related Concept Videos
Diversity of Archaea IV
Hyperthermophilic Bacteria
Microbes and Methanogenesis
Microbial Interactions: Mutualism
Diversity of Archaea I
Carbon-dioxide Fixation

