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Acetate production by methanogenic bacteria.

P Westermann1, B K Ahring, R A Mah

  • 1Division of Environmental and Occupational Health Sciences, School of Public Health, University of California at Los Angeles, Los Angeles, California 90024.

Applied and Environmental Microbiology
|September 1, 1989
PubMed
Summary

Methanosarcina barkeri and Methanosarcina mazei produce acetate during growth. However, Methanosarcina barkeri generates significantly more acetate than Methanosarcina mazei, despite similar methane yields.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Methanosarcina species are crucial methanogens in various environments.
  • Acetate is a key metabolic byproduct in microbial ecosystems.
  • Understanding differential metabolic outputs is vital for microbial ecology.

Purpose of the Study:

  • To compare acetate production in two Methanosarcina species.
  • To investigate the influence of different substrates on acetate formation.
  • To quantify acetate yield relative to methane production.

Main Methods:

  • Culturing of Methanosarcina barkeri and Methanosarcina mazei on H(2)-CO(2), methanol, and trimethylamine.
  • Measurement of acetate production.
  • Quantification of methane (CH(4)) formation and cell yields.

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Main Results:

  • Both Methanosarcina barkeri and Methanosarcina mazei produced acetate across all tested substrates.
  • Methanosarcina barkeri exhibited substantially higher acetate production (30–75 µmol acetate/mmol CH(4)) compared to Methanosarcina mazei (8–9 µmol acetate/mmol CH(4)).
  • Methane and cell yields were comparable between the two species, highlighting differences in acetate metabolism.

Conclusions:

  • Significant interspecies variation exists in acetate production within the Methanosarcina genus.
  • Methanosarcina barkeri is a more prolific acetate producer than Methanosarcina mazei under the tested conditions.
  • These findings have implications for understanding carbon cycling in methanogenic environments.