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Nitrogen Fixation in Continuous Culture with NH(4)Cl-Containing Media.

C Fritzsche1, E G Niemann

  • 1Institute of Biophysics, University of Hannover, Herrenhaeuser Strasse 2, 3000 Hannover 21, Federal Republic of Germany.

Applied and Environmental Microbiology
|April 1, 1990
PubMed
Summary

Azospirillum brasilense and other nitrogen-fixing bacteria can simultaneously use ammonium and nitrogen gas as sources. This simultaneous use occurs even under carbon-limited conditions, indicating efficient nitrogen assimilation in these microbes.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Nitrogen-fixing microorganisms play a crucial role in global nitrogen cycling.
  • Understanding the nitrogen assimilation pathways in bacteria is essential for agricultural and environmental applications.
  • Azospirillum brasilense is a well-studied nitrogen-fixing bacterium.

Purpose of the Study:

  • To investigate the nitrogen (N) balance and N dilution in Azospirillum brasilense Sp7 and two unidentified gram-negative nitrogen-fixing microorganisms.
  • To determine if these organisms can utilize both ammonium (NH4Cl) and molecular nitrogen (N2) simultaneously under specific growth conditions.

Main Methods:

  • Continuous culture experiments were performed to monitor microbial growth.
  • Nitrogen balance and dilution were measured at different ammonium chloride (NH4Cl) concentrations (1.1 and 2.2 mM).

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  • Carbon-limited conditions were maintained throughout the experiments.

    • Main Results:

    • Organisms utilized both NH4Cl and N2 simultaneously as nitrogen sources under carbon limitation.
    • Simultaneous nitrogen source utilization was observed at 1.1 mM and 2.2 mM NH4Cl steady states.
    • No detectable ammonium was found in the culture supernatant, suggesting complete assimilation.

    Conclusions:

    • Azospirillum brasilense and related bacteria can efficiently co-utilize inorganic and molecular nitrogen sources.
    • Complete assimilation of available nitrogen sources under carbon limitation highlights metabolic flexibility.
    • These findings have implications for understanding microbial nitrogen metabolism and its ecological significance.