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Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
Published on: April 10, 2016
Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Pseudomonas fluorescens Proteases in
R S Clements1, D M Wyatt, M H Symons
1Immunology Department, Queensland University of Technology, Brisbane, 4000, and Department of Primary Industries, Queensland Food Research Laboratories, Hamilton, 4007, Queensland, Australia.
Abstract:
An inhibition enzyme-linked immunosorbent assay was developed to detect low levels of the proteases extracted from four strains of Pseudomonas fluorescens. The assay detected between 0.24 and 7.8 ng of protease per ml of ultrahigh-temperature-treated milk and could be completed within 6 h. It could be used as a framework for a test system for quantifying spoilage proteases in dairy products.
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