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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri
1Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695.
Applied and Environmental Microbiology
|April 1, 1990
Abstract:
A coenzyme B(12)-dependent glycerol dehydratase from Lactobacillus reuteri has been purified and characterized. The dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. K(m) values for substrates and coenzyme B(12) were in the millimolar and the submicromolar range, respectively.
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