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Updated: Aug 14, 2026

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Detection of Protease Activity by Fluorescent Peptide Zymography
Published on: January 20, 2019
Simple, sensitive zymogram technique for detection of xylanase activity in polyacrylamide gels
1Department of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210.
Applied and Environmental Microbiology
|June 1, 1990
Abstract:
A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background.

