Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mannanase Components from Bacillus pumilus.

A Araujo1, O P Ward

  • 1Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Applied and Environmental Microbiology
|June 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient experience preparing for prostate cancer radiotherapy.

Technical innovations & patient support in radiation oncology·2025
Same author

Management of infusion-related reactions in cancer therapy: strategies and challenges.

ESMO open·2024
Same author

Inferring the diurnal variability of OH radical concentrations over the Amazon from BVOC measurements.

Scientific reports·2023
Same author

Rare RNF213 variant in adolescent with moyamoya disease.

Revista de neurologia·2023
Same author

Unresectable stage III non-small cell lung cancer: Insights from a Portuguese expert panel.

Pulmonology·2023
Same author

Lung cancer survival and sex-specific patterns in Portugal: A population-based analysis.

Pulmonology·2021
Same journal

A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

Applied and environmental microbiology·2026
Same journal

Sources and traits of bacteria and fungi found in the near-surface atmosphere.

Applied and environmental microbiology·2026
Same journal

Advancing One Health in Africa through continental early warning environmental surveillance.

Applied and environmental microbiology·2026
Same journal

Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

Applied and environmental microbiology·2026
Same journal

Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

Applied and environmental microbiology·2026
Same journal

Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

Applied and environmental microbiology·2026
See all related articles

Two beta-d-mannanase components (A and B) from Bacillus pumilus were purified. Component B showed significantly higher specific activity than component A, indicating distinct enzymatic properties for these mannanase enzymes.

Area of Science:

  • Biochemistry
  • Enzymology
  • Microbiology

Background:

  • Beta-d-mannanase enzymes are crucial for degrading mannan polysaccharides.
  • Bacillus pumilus is a known source of industrially relevant enzymes.
  • Enzyme purification is essential for characterizing specific enzymatic properties.

Purpose of the Study:

  • To purify and characterize beta-d-mannanase from Bacillus pumilus.
  • To investigate the distinct properties of different enzyme components.
  • To assess the potential of purified enzymes for biotechnological applications.

Main Methods:

  • Purification of beta-d-mannanase from Bacillus pumilus using biochemical techniques.
  • Homogeneity assessment via polyacrylamide gel electrophoresis (PAGE).

Related Experiment Videos

  • Characterization of enzyme components including molecular weight, carbohydrate content, specific activity, pH optimum, and thermal stability.

    • Main Results:

    • Two homogeneous beta-d-mannanase components, A and B, were isolated.
    • Component A: MW 55,000, 15.3% carbohydrate, specific activity 78 U/mg, pH optimum 5.5-6.9, half-life 60 min at 70°C.
    • Component B: MW 37,000, 7.2% carbohydrate, specific activity 1,616 U/mg, pH optimum 6.0, half-life 21 min at 70°C.

    Conclusions:

    • Bacillus pumilus produces at least two distinct beta-d-mannanase components with differing biochemical properties.
    • Component B exhibits significantly higher specific activity compared to component A.
    • These findings highlight the heterogeneity of beta-d-mannanase and provide insights into enzyme optimization for industrial use.