Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

DNA Probe for Lactobacillus delbrueckii.

M Delley¹, B Mollet, H Hottinger

¹Nestec Ltd., Nestlé Research Centre, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.

Applied and Environmental Microbiology

|June 1, 1990

Summary

Researchers identified a specific DNA probe from Lactobacillus delbrueckii subsp. bulgaricus. This probe accurately identifies key Lactobacillus species, aiding in bacterial strain differentiation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Commensal Bifidobacterium longum Strain Prevents Gluten-Related Immunopathology in Mice through Expression of a Serine Protease Inhibitor.

Applied and environmental microbiology·2017

Same author

Putative frameshift suppressors in Schizosaccharomyces pombe.

Current genetics·2013

Same author

Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.

Applied and environmental microbiology·2006

Same author

Heterologous expression and characterization of the exopolysaccharide from Streptococcus thermophilus Sfi39.

European journal of biochemistry·2001

Same author

Transformation of Endomyces fibuliger based on its gene for orotidine-5'-phosphate decarboxylase.

Gene·2000

Same author

Genomics, molecular genetics and the food industry.

Journal of biotechnology·2000

Area of Science:

Microbiology
Molecular Biology
Genomics

Background:

Accurate identification of bacterial species is crucial for various applications, including food science and diagnostics.
Traditional methods for bacterial identification can be time-consuming and may lack specificity.
Development of molecular tools offers a more precise and efficient approach to bacterial characterization.

Purpose of the Study:

To isolate and characterize a DNA fragment from Lactobacillus delbrueckii subsp. bulgaricus with potential as a specific probe.
To evaluate the specificity and sensitivity of the isolated DNA probe for identifying different Lactobacillus strains.
To develop a molecular tool for the reliable detection of specific Lactobacillus species.

Main Methods:

Construction and screening of a genomic DNA library from Lactobacillus delbrueckii subsp. bulgaricus.
Complementation of a leucine auxotrophy in an Escherichia coli strain (GE891) to identify functional clones.
Dot-blot hybridization assays using the isolated DNA clone as a probe against a panel of over 40 Lactobacillus strains.
Sensitivity testing using an alpha-P-labeled DNA probe.

Main Results:

A specific DNA clone was successfully isolated from Lactobacillus delbrueckii subsp. bulgaricus.
The isolated DNA clone demonstrated high specificity, recognizing Lactobacillus delbrueckii subsp. delbrueckii, bulgaricus, and lactis.
Dot-blot hybridizations confirmed the probe's ability to differentiate between these target species and other tested Lactobacillus strains.
The DNA probe exhibited good sensitivity in detecting target bacteria.

Conclusions:

The isolated DNA clone serves as a specific and sensitive DNA probe for the identification of Lactobacillus delbrueckii subsp. delbrueckii, bulgaricus, and lactis.
This molecular tool can significantly improve the accuracy and efficiency of Lactobacillus strain identification.
The findings provide a valuable resource for microbial diagnostics and strain typing in research and industry.

Related Experiment Videos