Related Experiment Video
Updated: Jul 22, 2026

Anaerobic Growth and Maintenance of Mammalian Cell Lines
Published on: July 21, 2018
Growth and Maintenance of Thiobacillus ferrooxidans Cells
1Department of Biological Sciences, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931.
Abstract:
A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture. Values determined for the optical densities at 500 nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate. The utility of this procedure was demonstrated by conducting physiological studies on the influence of CO(2) and FeSO(4) availability on the growth of T. ferrooxidans. In addition, we describe a procedure for the long-term maintenance of cells T. ferrooxidans that ensures culture purity and genetic stability.
Related Concept Videos
Microbial Nutrition
Microbial Fermentation
Oxygen Requirements and Growth Patterns
Anoxygenic Phototrophic Bacteria
Hyperthermophilic Bacteria
Microbes and Other Elemental Cycles

