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Binding of Dissolved Strontium by Micrococcus luteus.

B D Faison1, C A Cancel, S N Lewis

  • 1Chemical Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6194.

Applied and Environmental Microbiology
|December 1, 1990
PubMed
Summary

Micrococcus luteus cells efficiently remove strontium (Sr) from water. This non-metabolic process involves Sr binding to the cell envelope, suggesting potential for bioremediation applications.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Bioremediation

Background:

  • Strontium (Sr) is a radioactive element with environmental implications.
  • Microbial biosorption offers a potential method for heavy metal removal from aqueous solutions.

Purpose of the Study:

  • To investigate the capacity and characteristics of strontium (Sr) removal by resting Micrococcus luteus cells.
  • To elucidate the mechanisms and location of Sr binding on the bacterial cell surface.

Main Methods:

  • Exposure of resting Micrococcus luteus cells to dilute strontium chloride (SrCl2) solutions.
  • Analysis of Sr binding capacity, kinetics, and influence of various cations and chemical treatments.
  • Localization of Sr binding activity on the bacterial cell envelope.

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Main Results:

  • Micrococcus luteus achieved high Sr loadings (25 mg Sr/g dry weight) from 50 ppm Sr solutions.
  • Sr binding was rapid, reversible, and did not require metabolic activity or nutrients.
  • Binding was inhibited by polyvalent cations and preferentially involved Ca and Sr; bound Sr was displaced by divalent ions or H+.

Conclusions:

  • Sr binding by Micrococcus luteus is a reversible equilibrium process.
  • Ion exchange via acidic cell surface components and potential intracellular uptake contribute to Sr binding.
  • The cell envelope is the primary site for Sr binding activity.