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Ornithine decarboxylase activity: control by cyclic nucleotides.

C V Byus, D H Russell

    Science (New York, N.Y.)
    |February 21, 1975
    PubMed
    Summary

    Exposure to cold and aminophylline increase cyclic adenosine monophosphate (cyclic AMP) in adrenal glands. This leads to higher ornithine decarboxylase activity, suggesting cyclic AMP regulates this enzyme synthesis.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Biochemistry

    Background:

    • Adrenal gland function is crucial for stress response.
    • Cyclic AMP (cAMP) acts as a second messenger in various cellular processes.
    • Ornithine decarboxylase (ODC) is a key enzyme in polyamine synthesis.

    Purpose of the Study:

    • To investigate the effect of cold exposure and aminophylline on adrenal gland cyclic AMP levels.
    • To determine the impact of these stimuli on ornithine decarboxylase activity.
    • To elucidate the role of cyclic AMP in regulating ODC activity in the adrenal gland.

    Main Methods:

    • Measurement of cyclic AMP levels in adrenal medulla and cortex following cold exposure and aminophylline administration.
    • Assay of ornithine decarboxylase activity in adrenal tissues.

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  • Analysis of enzyme synthesis in response to stimuli.

    • Main Results:

    • Both cold exposure and aminophylline significantly increased cyclic AMP levels in the adrenal medulla and cortex.
    • A subsequent dramatic increase in ornithine decarboxylase activity was observed.
    • Evidence suggests this increase in ODC activity is due to de novo enzyme synthesis.

    Conclusions:

    • Cyclic adenosine monophosphate (cyclic AMP) plays a regulatory role in adrenal ornithine decarboxylase activity.
    • Cold stress and aminophylline-induced elevations in cyclic AMP trigger increased synthesis of ornithine decarboxylase.
    • These findings highlight a signaling pathway linking environmental/pharmacological stimuli to gene expression in the adrenal gland.