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Updated: Aug 14, 2026

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
Published on: March 23, 2018
Improved technique for identification and enumeration of methanogenic bacterial colonies on roll tubes by
N Kataoka1, Y Tokiwa, K Takeda
1Department of Development and Planning, EBARA Corporation, 1-6-27, Khonan, Minato-ku, Tokyo 108, and Chemical Ecology Division, Fermentation Research Institute, Agency of Industrial Science and Technology, MITI, 1-1-3, Higashi, Tsukuba, Ibaraki 305, Japan.
Abstract:
Methanogenic fluorescent colonies can be clearly identified on roll tubes by using an epifluorescence microscope equipped with a x 2 objective. Methanogenic and nonmethanogenic colonies could be counted in roll tubes prepared from methanogenic enrichment cultures. Late-developing colonies appearing after 25 days of incubation were mainly methanogenic.

