Molecular Cloning of the Tryptophan Operon from an Aeromonas hydrophila Freshwater Isolate
J Anguita1, O Rivero, G Naharro
1Departamento de Patología Animal (Sanidad Animal), Unidad de Microbiología, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
Abstract:
A genomic library of Aeromonas hydrophila F9 was constructed by using pBR322 as a vector. From that, two DNA fragments (5.8 and 11.6 kb) were isolated containing genetic information to complement trpA and trpB defects (5.8-kb fragment) and to complement trpA, trpB, trpC, trpD, and trpE defects (11.6-kb fragment) in Escherichia coli mutants. Evidence of the existence of a secondary promoter is given.
More Related Videos
12:29Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
Published on: March 11, 2022
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
Related Concept Videos
Repressible Operon: trp Operon
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
