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Related Experiment Videos

Isolation and characterization of a mo -reducing bacterium.

B Ghani1, M Takai, N Z Hisham

  • 1Department of Biotechnology, Universiti Pertanian Malaysia, 43400 Serdang, Selangor, Malaysia.

Applied and Environmental Microbiology
|April 1, 1993
PubMed
Summary

A newly identified bacterium, Enterobacter cloacae strain 48, reduces molybdate to molybdenum blue using glucose metabolism and electron transport. This microbial reduction process offers potential for bioremediation applications.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Molybdenum (Mo) is an essential trace element with various industrial applications.
  • Microbial reduction of heavy metals is a key process in biogeochemical cycles and bioremediation.
  • The specific mechanisms of microbial molybdate reduction are not fully understood.

Purpose of the Study:

  • To isolate and characterize a novel molybdate-reducing bacterium.
  • To investigate the metabolic pathways and electron donors involved in microbial Mo reduction.
  • To explore the potential of this bacterium for molybdenum transformation.

Main Methods:

  • Isolation and identification of a molybdate-reducing bacterium (strain 48) from Malaysian stream water.
  • Cultivation under anaerobic conditions with glucose-yeast extract medium and molybdate.

Related Experiment Videos

  • Analysis of Mo reduction products (molybdenum blue) and percentage of Mo reduced.
  • Enzymatic assays using inhibitors (iodoacetic acid, sodium fluoride, sodium cyanide) and electron donors (NADH, N,N,N',N'-tetramethyl-p-phenylenediamine).
  • Spectrophotometric analysis of cytochrome b reduction and effect of metal ions (ferric, stannous).

    • Main Results:

    • Strain 48, identified as Enterobacter cloacae, effectively reduced molybdate to molybdenum blue.
    • Approximately 27% of Mo was reduced within 28 hours.
    • Mo reduction was linked to the glycolytic pathway and electron transport, inhibited by specific metabolic inhibitors.
    • NADH and N,N,N',N'-tetramethyl-p-phenylenediamine served as effective electron donors.
    • Ferric and stannous ions significantly enhanced NADH-dependent Mo reduction.
    • Cytochrome b reduction preceded molybdenum blue formation, with downstream components of the electron transport chain acting as electron donors.

    Conclusions:

    • Enterobacter cloacae strain 48 is a capable molybdate-reducing bacterium.
    • Microbial Mo reduction is an energy-dependent process involving glycolysis and the electron transport chain.
    • The findings provide insights into the biochemical mechanisms of microbial Mo reduction and its potential environmental significance.