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Updated: Aug 14, 2026
Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Genetic and Biochemical Characterization of Nectria haematococca Strains with Adhesive and Adhesion-Reduced
L Epstein1, Y H Kwon, D E Almond
1Division of Entomology, Plant and Soil Microbiology, Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720.
Abstract:
A previous study reported the isolation of two mutants (LE1 and LE2) of the plant pathogenic fungus Nectria haematococca (anamorph, Fusarium solani f. sp. cucurbitae) with macroconidia with reduced ability to adhere (Att) to zucchini fruits and polystyrene. The adhesion-reduced-phenotype in LE1 and LE2 macroconidia is temperature sensitive and dependent on the concentration of nutrients. Classical genetic analysis of progeny derived from LE1 identified a mutation in a genetic locus, named Att1. The 90-kDa glycoprotein and macroconidial tip mucilage which were previously associated with the development of adhesion competence in Att macroconidia are specifically associated with macroconidia; neither is produced on microconidia, which are relatively nonadherent. However, macroconidia of both Att and Att strains produce the 90-kDa glycoprotein and the macroconidial tip mucilage.
