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Field evaluation of two colorimetric coliphage detection methods.

M M Ijzerman1, J O Falkinham, R B Reneau

  • 1Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061.

Applied and Environmental Microbiology
|March 1, 1994
PubMed
Summary

Two new colorimetric methods for coliphage detection, the colorimetric agar-based (CAB) and liquid colorimetric presence-absence (LCPA) methods, are as sensitive and reliable as the standard American Public Health Association (APHA) method.

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Area of Science:

  • Environmental microbiology
  • Water quality assessment
  • Bacteriophage detection

Background:

  • Coliphages are indicators of fecal contamination in water.
  • Current detection methods can be labor-intensive and time-consuming.
  • Development of rapid and reliable detection assays is crucial for public health.

Purpose of the Study:

  • To compare the efficacy of two novel colorimetric methods (CAB and LCPA) for coliphage detection against the established APHA standard method.
  • To evaluate the sensitivity, ease of use, and reliability of the new methods.

Main Methods:

  • Comparison of colorimetric agar-based (CAB) and liquid colorimetric presence-absence (LCPA) methods with the American Public Health Association (APHA) standard method.
  • Assay principle: Induction of beta-galactosidase in Escherichia coli followed by enzymatic cleavage of a chromogenic substrate.

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  • Testing of 90 split water samples from diverse sources.

    • Main Results:

    • The CAB and LCPA methods demonstrated comparable sensitivity to the APHA method in coliphage detection.
    • Both new methods were found to be easier to read and interpret than the APHA method.
    • The LCPA method proved to be as reliable and sensitive as agar-based methods, offering a rapid and simple alternative.

    Conclusions:

    • The CAB and LCPA methods offer sensitive and reliable alternatives for coliphage detection.
    • These novel methods provide advantages in terms of ease of interpretation and speed, particularly the LCPA method.
    • The findings support the adoption of these colorimetric assays for routine water quality monitoring.