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Isolation of a Bacterial Culture That Degrades Methyl t-Butyl Ether

J P Salanitro1, L A Diaz, M P Williams

  • 1Shell Development Company, Westhollow Research Center, Houston, Texas 77251-1380.

Applied and Environmental Microbiology
|July 1, 1994
PubMed

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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...