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Updated: Aug 14, 2026
09:10
Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
Published on: May 27, 2015
Detection of beta-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate
1Department of Microbiology, Seoul National University, Seoul, 151-742, Korea.
Applied and Environmental Microbiology
|December 1, 1994
Abstract:
beta-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by beta-glucosidase action reacts with ferric ion to produce a black band, corresponding to the beta-glucosidase, against the transparent background.
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