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The Lambda Select cII Mutation Detection System
Published on: April 26, 2018
Differential Gene Expression in the Laccase Gene Family from Basidiomycete I-62 (CECT 20197)
M Mansur1, T Suárez, A E González
1Departamento de Microbiologia Molecular, Centro de Investigaciones Biológicas del Consejo Superior de Investigaciones Cientificas, 28006 Madrid, and Centro Nacional de Biotecnologia, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain, and Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar, Havana, Cuba.
Abstract:
A family of genes encoding laccases has recently been described for the basidiomycete I-62 (CECT 20197). Transcript levels of genes lcc1, lcc2, and lcc3 were analyzed under four different culture conditions to study their expression patterns. Two of the laccase genes were clearly inducible by veratryl alcohol: the lcc1 gene is inducible in early stages of growth, and the lcc2 gene is also inducible but only when the organism reaches the stationary phase. Transcript levels for the third gene, lcc3, were uninduced by veratryl alcohol and repressed by glucose.
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